instrument301 is an automation solution for processing microarrays or blot membranes. This includes all washing steps and downstream reactions, e.g. incubation with a secondary or tertiary antibody. Each unit can process 1–4 microarrays or small membranes (slide format) or two medium-sized or one large membrane (max. 13.5 x 8.0 cm). 5 units can be connected to extend the processing capacity to 20 slides or 5 large membranes.
Accurate, non-contact addition of all liquids minimizes sample carryover between individual processing steps improving the washing efficiency and reducing the overall processing time. The liquid is driven through the supply line with a regulated positive pressure and a high-quality pinch valve controls the flow.
The Immune Proteomics group of the Medizinisches Proteom-Center (Medical Proteom-Center) of the Ruhr-University Bochum, Germany uses 5 instrument301 to process up to 20 Human ProtoArrays® V 5.0 (Life Technologies, USA) or self-made protein microarrays in one run. In these experiments human sera are screened for disease specific autoantibodies, which are detected by a secondary, fluorescently labeled antibody.
All units are PC controlled and include a three channel on demand non-contact liquid supply, a liquid removal, Peltier driven cooling, orbital shaking as well as tilting of the slide tray to enable quantitative removal of the liquid past each processing step. A typical experiment includes slide blocking, a brief wash, serum incubation, 5 washes, incubation with the secondary antibody followed by another 5 washes.
Incubation times, volumes and temperatures can be individually set within the instrument control software. An intuitive graphical user interface allows setting up a complete new method or modifying an existing run by a few simple mouse clicks. Smart wizards and automation scripts take care of washing, cleaning and disinfection of all components.
1 unit stand alone;
W 22cm, D 40cm, H 18cm