Instrument301 is an automation solution for slide processing (3 x 1 inch; 75 x 25 mm) including washing and downstream incubation, e.g. with a secondary antibody. Each unit can process 1-4 slides and 2, 3, 4 or more units can be connected to extend the processing capacity.
i301 - instrument301 Flyer PDF
4 Compartments - Dispense, - Shake, - Cool, - Flush
Picture shows 5 units of instrument301
Picture shows close-up of instrument301
The Immune Proteomics group of the Medizinisches Proteom-Center of the Ruhr-University Bochum, Germany uses 5 instrument301 to process up to 20 Human ProtoArrays® V 5.0 (Life Technologies, USA) or self-made protein microarrays in one run. In these experiments human sera are screened for disease specific autoantibodies, which are detected by a secondary, fluorescently labeled antibody.
All units are PC controlled and include a three channel on demand non-contact liquid supply, a liquid removal, Peltier driven cooling, orbital shaking as well as tilting of the slide tray to enable quantitative removal of the liquid past each processing step. A typical experiment includes slide blocking, a brief wash, serum incubation, 5 washes, incubation with the secondary antibody followed by another 5 washes. Incubation times, volumes and temperatures can be individually set within the instrument control software. An intuitive graphical user interface allows setting up a complete new method or modifying an existing run by a few simple mouse clicks. Smart wizards and automation scripts take care of washing, cleaning and disinfection of all components.